《植物生理学报》 2014, 50(2): 197-202

粗枝云杉胚性愈伤组织增殖后期的体细胞胚发生方式转变

张建伟¹, 王军辉^1,*, 马建伟²

¹中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京100091; ²甘肃省小陇山林业科学研究所, 甘肃天水741022

通信作者：王军辉；E-mail: wangjh@caf.ac.cn；Tel: 010-62888539

摘　要：

粗枝云杉愈伤组织在增殖后期体细胞胚的分化能力显著降低, 转变愈伤组织增殖方式和体细胞胚分化培养方式有利于体细胞胚发生能力的提高。采用液体悬浮增殖取代半固体增殖更有利于胚性的保持。在增殖后期, 首选的体细胞胚发生方式为“液体增殖-滤纸分化”, 其次为“块状增殖-块状分化”, 最后是“块状增殖-滤纸分化”。

关键词：粗枝云杉; 胚性愈伤组织; 增殖时期; 体细胞胚胎发生

收稿：2013-09-11   修定：2014-01-13

资助：“十二五”国家科技支撑项目(2012BAD01B01)

The Way Change of Somatic Embryogenesis at the Late Stage of Embryogenic Callus Proliferation of Picea asperata Mast

ZHANG Jian-Wei¹, WANG Jun-Hui^1,*, MA Jian-Wei²

¹Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; ²Xiaolongshan Forestry Research Institute, Tianshui, Gansu 741022, China

Corresponding author: WANG Jun-Hui; E-mail: wangjh@caf.ac.cn; Tel: 010-62888539

Abstract:

Somatic embryogenesis of Picea asperata decreased significantly (P<0.01) as the time extension. At the late proliferation stage, changing the ways of proliferation and maturation could significantly increase the capacity for somatic embryogenesis. Compared with semi-solid culture, suspension culture was more conducive to retention of embryogenesis. At the late stage of proliferation, the optimal maturation approach was suspension culture-paper differentiation, chunk culture-chunk differentiation came in second, followed by chunk culture-paper differentiation.

Key words: Picea asperata; embryogenic callus; proliferation stage; somatic embryogenesis